目錄:亞科因（武漢）生物技術有限公司>>樣品制備及檢測>>WB/IP/Co-IP/IF工具箱>> IPKine™ Anti-HA Magnetic IP KitIPKine™ HA標簽蛋白免疫沉淀試劑盒（磁珠法）
| 產(chǎn)品英文名稱 | IPKine™ Anti-HA Magnetic IP Kit |
| 產(chǎn)品中文名稱 | IPKine™ HA標簽蛋白免疫沉淀試劑盒（磁珠法） |
| 免疫原 | 合成多肽 |
| 試劑盒組分 | Non-Denaturing Lysis Buffer TBS (10×) Anti-HA Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer HA Peptide (25×) SDS-PAGE Loading Buffer (5×) |
| 特點&優(yōu)勢 | ? 高效：特異性強、靶蛋白結(jié)合量高，≥0.6 mg HA標簽融合蛋白/mL磁珠； ? 便捷：可結(jié)合多種形式的HA標簽蛋白（N端HA融合蛋白、C端HA融合蛋白）； ? 通用：提供IP實驗所需的所有必要緩沖液； ? 可靠：提供陰性對照，可排除IgG本身和目的蛋白或其它特定生物分子的非特異性結(jié)合； ? 靈活：本試劑盒提供三種洗脫方法（HA多肽競爭洗脫、酸洗脫和SDS-PAGE Loading Buffer洗脫方法）。 |
| 保存建議 | 按各組分標簽提示分開存儲，保質(zhì)期12個月。 |
| 運輸條件 | 冰袋運輸（藍冰） |
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| 背景 | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. |
Figure. The immunoprecipitation effect of Anti-HA Magnetic IP Kit used for HA-Tag fusion protein. HEK293T cells were transfected with HA-Tag plasmid. Lane 1 was whole cell lysate (WCL); Lane 2 was the immunoprecipitation sample of Mouse IgG Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer; Lane 3 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer. Lane 4 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by Elution Buffer; Lane 5 was the immunoprecipitation sample of Anti-HA Magnetic Beads eluted by Working HA Peptide. By using peptide elution and acid elution, only contained HA-Tag fusion protein, did not contain heavy and light chains of antibody.
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