AAT Bioquest-XFD488化學(xué)結(jié)構(gòu)與Alexa Fluor®488相同（Alexa Fluor™是ThermoFisher的商標(biāo)）。麥胚凝集素（WGA）是一種與N-乙酰-D-葡糖胺和唾液酸結(jié)合的凝集素。由于其生物學(xué)應(yīng)用，它是研究最多、最有用的凝集素之一。由于WGA與糖復(fù)合物結(jié)合，其衍生物和偶聯(lián)物被廣泛用于標(biāo)記細(xì)胞膜和纖維化瘢痕組織，用于熒光成像和分析。WGA的碳水化合物結(jié)合特異性針對(duì)β-1,4-GlcNAc連接的殘基序列，即殼聚糖。每個(gè)單體包含兩個(gè)相同的、不相互作用的結(jié)合位點(diǎn)，它們與3或4個(gè)β-1,4-GlcNAc單元互補(bǔ)。在所研究的單糖中，只有GlcNAc與WGA結(jié)合。ManNAc不結(jié)合，GalNAc只弱結(jié)合。WGA的XFD488共軛物相當(dāng)于WGA的Alexa Fluor®488共軛物。它表現(xiàn)出明亮的綠色熒光。XFD488 WGA綴合物與唾液酸和N-乙酰氨基葡萄糖殘基結(jié)合。

艾美捷Wheat Germ Agglutinin, AF488 Labeled小麥胚芽凝集素，AF488標(biāo)記：

貨號(hào)：AAT-25500

修正因子（260納米）：0.30

修正因子（280納米）：0.11

消光系數(shù)（厘米^-1 摩爾^-1）：71000

激發(fā)（納米）：499

發(fā)射（納米）：520

量子產(chǎn)率：0.921

預(yù)期用途：僅限研究使用（RUO）

儲(chǔ)存：冷凍（< -15°C）；盡量減少光照

熒光顯微鏡

激發(fā) FITC濾光片組

發(fā)射 FITC濾光片組

推薦板 黑色壁，透明底部

圖：活HeLa細(xì)胞用5µg/mL標(biāo)記的小麥胚凝集素XFD488染色30分鐘，然后用Hoechst 33342（目錄號(hào)17535）染色。使用FITC和DAPI濾光片組通過熒光顯微鏡獲取圖像。

Wheat Germ Agglutinin, AF488 Labeled小麥胚芽凝集素，AF488標(biāo)記文獻(xiàn)參考：

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.

Authors: Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida

Journal: Plant physiology and biochemistry : PPB (2020): 420-427

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.

Authors: Jankovi?, Tamara and Go?, Sanja and Miti?, Ninoslav and Danilovi? Lukovi?, Jelena and Jankovi?, Miroslava

Journal: Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.

Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J

Journal: mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.

Authors: Killilea, David W and McQueen, Rebecca and Abegania, Judi R

Journal: Journal of food science (2020): 808-815

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.

Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu

Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572