化工儀器網(wǎng)
詳細(xì)介紹
細(xì)胞實(shí)時(shí)形變測(cè)量分析系統(tǒng)，Real-Time Deformability Cytometry
來(lái)自德國(guó)的高通量單細(xì)胞形變測(cè)量分析系統(tǒng)
該系統(tǒng)是一套基于微流控流體壓力梯度的、在倒置顯微鏡的擴(kuò)展起來(lái)的、集成流式細(xì)胞儀特性、熒光檢測(cè)模塊、溫控模 塊、高速成像和數(shù)據(jù)采集分析軟件的高通量單細(xì)胞實(shí)時(shí)形變測(cè)量和單細(xì)胞力學(xué)性質(zhì)分析系統(tǒng)。
是一種以流式細(xì)胞儀的速度檢測(cè)單個(gè)細(xì)胞形態(tài)和力學(xué)性質(zhì)的技術(shù)，每個(gè)細(xì)胞都被實(shí)時(shí)拍攝、分析和成像存儲(chǔ)。 此外，非破壞性的力量應(yīng)用于細(xì)胞，提供一種方便，穩(wěn)健和高通量的技術(shù)進(jìn)行生物標(biāo)志物的檢測(cè)，可用于基礎(chǔ)科學(xué)和臨床研究。
探索細(xì)胞的物理特性作為生物標(biāo)志物,可以將非破壞性的力量應(yīng)用于細(xì)胞或珠子，并觀察它們的變形。 這允許研究對(duì)物理壓力的特定機(jī)械響應(yīng)。
優(yōu)勢(shì)亮點(diǎn)：
- 機(jī)械力學(xué)作為一種新的生物標(biāo)志物--溫和無(wú)損傷
- 無(wú)標(biāo)記
- 非破壞性的力量
- 高速測(cè)量單個(gè)細(xì)胞的實(shí)時(shí)形變（高度、寬度、面積、比例）、高度偏差、環(huán)度衡量比、孔度、亮度、楊氏模量等
- 細(xì)胞機(jī)械特性測(cè)量高通量（1000細(xì)胞/秒）
- 配有高速成像、熒光檢測(cè)、溫控模塊
- 不需要細(xì)胞分離/純化
- 文獻(xiàn)量大、級(jí)別高文章達(dá)數(shù)十篇
實(shí)時(shí)形變
成像
每個(gè)細(xì)胞被同時(shí)拍照、分析和儲(chǔ)存。 這允許通過(guò)它們的光學(xué)特性來(lái)找到小亞群或區(qū)分細(xì)胞。 另外可以研究像表面拓?fù)浠蚣?xì)胞對(duì)光的衰減的形態(tài)特性。
- 每個(gè)獲取圖像的存儲(chǔ)
- 快速訪問(wèn)細(xì)胞大小和形態(tài)
流式細(xì)胞技術(shù)
細(xì)胞通過(guò)微流通道時(shí),提取細(xì)胞變形、亮度和大小等參數(shù)，同時(shí)。 這允許實(shí)時(shí)地研究細(xì)胞屬性。
可溫控和熒光檢測(cè)
實(shí)時(shí)變形細(xì)胞計(jì)數(shù)和同時(shí)熒光檢測(cè)：
- 熒光模塊使得該系統(tǒng)不再只是附加了一個(gè)額外的細(xì)胞力學(xué)檢測(cè)通道的流式細(xì)胞儀。它成為了生命科學(xué)實(shí)驗(yàn)室的得力工具 - 提供了更多視角來(lái)解決科學(xué)問(wèn)題。在生物學(xué)研究中通常使用熒光流式細(xì)胞儀來(lái)鑒定和定量細(xì)胞和細(xì)胞過(guò)程。使該系統(tǒng)集熒光流式細(xì)胞儀和實(shí)時(shí)變形的優(yōu)點(diǎn)于一身，形成了實(shí)時(shí)熒光形變細(xì)胞儀。光片激發(fā)設(shè)計(jì)可實(shí)現(xiàn)三通道1D熒光成像。除了所有實(shí)時(shí)變形參數(shù)外，系統(tǒng)還會(huì)分析熒光信號(hào)實(shí)時(shí)得到峰圖，速度可達(dá)每秒1000個(gè)細(xì)胞。也可在實(shí)驗(yàn)后處理保存的原始熒光數(shù)據(jù)，以針對(duì)特定的問(wèn)題和需 求修改處理方法。
1)根據(jù)表面marker鑒定血細(xì)胞：
熒光模塊可檢測(cè)和鑒定同一樣品中的三種不同熒光。利用標(biāo)記的表面熒光蛋白可同時(shí)實(shí)現(xiàn)細(xì)胞鑒定和力學(xué)性質(zhì)及形態(tài)性質(zhì)測(cè)量。 下圖為 G-CSF動(dòng)員的外周血樣品細(xì)胞群體。 標(biāo)記后的細(xì)胞表面markers CD3-FITC (T-cells)， CD34-PE (造血干細(xì)胞)和CD14-APC(單核細(xì)胞)熒光強(qiáng)度檢測(cè) 揭示了各細(xì)胞類型所具有的不同力學(xué)性質(zhì)。- 2)一維熒光成像：
熒光模塊在激發(fā)光路徑中產(chǎn)生一束受限光片，穿過(guò)流道，細(xì)胞會(huì)經(jīng)過(guò)一束很窄的激發(fā)光幕。這樣可以進(jìn)行1D熒光成像，例如可用于解析沿流動(dòng)方向的熒光標(biāo)記結(jié)構(gòu)的側(cè)向分布。檢測(cè)到的熒光峰值帶有很多重要信息。熒光標(biāo)記的胞內(nèi)結(jié)構(gòu)（如細(xì)胞核）會(huì)顯示窄峰，而胞質(zhì)會(huì)顯示出更寬的峰。不同分裂期細(xì)胞中標(biāo)記的組蛋白也會(huì)呈現(xiàn)出不同的峰圖.
加熱模塊 - 溫度控制
加熱模塊實(shí)現(xiàn)了生理溫度下的測(cè)量。加熱模塊帶有一個(gè)300 W的加熱器和幾個(gè)靜默通風(fēng)機(jī)來(lái)有效混合熱空氣?？拷鼧悠诽幱幸粋€(gè)傳感器和一個(gè)控制單元，用以地將溫度控制在所需值。系統(tǒng)的空氣循環(huán)系統(tǒng)非常高效，當(dāng)進(jìn)行開放操作（如更換樣品）后可以迅速恢復(fù)溫度。
高速攝像
該成像模塊是款高速明場(chǎng)攝像顯微鏡,使用同步化微秒高強(qiáng)度LED光源減輕運(yùn)動(dòng)模糊,可進(jìn)行極慢運(yùn)動(dòng)攝影.每秒可記錄500幅全幀圖像或10000幀小區(qū)域圖像
越來(lái)越多的研究表明，機(jī)械力(Mechanical Forces)是機(jī)體生長(zhǎng)發(fā)育、結(jié)構(gòu)重建以及功能維持的重要因素，也與疾病的發(fā)生發(fā)展密切相關(guān)。力學(xué)刺激(Mechanical Stimulation)會(huì)對(duì)細(xì)胞的粘附、鋪展、遷移、增殖和分化等行為有著重要的影響，力學(xué)表型(Mechanical phenotype)的改變也可用來(lái)定量檢測(cè)、評(píng)價(jià)細(xì)胞的生物學(xué)功能。力學(xué)生物學(xué)(Mechnobiology)研究對(duì)深入揭示疾病機(jī)理、發(fā)展新的診斷治療方法具有重要意義，還可以為生物材料、再生醫(yī)學(xué)、藥物篩選等應(yīng)用研究提供新思路、新工具。由于細(xì)胞內(nèi)在的生物復(fù)雜性及微納米尺度的制約，如何實(shí)現(xiàn)細(xì)胞及亞細(xì)胞水平上力學(xué)信息的實(shí)時(shí)、原位、動(dòng)態(tài)探測(cè)和操控是力學(xué)生物學(xué)中的挑戰(zhàn)性的問(wèn)題之一。
| Zebrafish spinal cord repair is accompanied by transient tissue stiffening S Möllmert, MA Kharlamova, T Hoche, AV Taubenberger, S Abuhattum, ... Biophysical Journal | 2 | 2019 |
| Polyacrylamide bead sensors for in vivo quantification of cell-scale stress in zebrafish development N Traeber, K Uhlmann, S Girardo, G Kesavan, K Wagner, J Friedrichs, ... Scientific reports 9 (1), 1-14 | 3 | 2019 |
| CASP1 variants influence subcellular caspase-1 localization, pyroptosome formation, pro-inflammatory cell death and macrophage deformability F Kapplusch, F Schulze, S Rabe-Matschewsky, S Russ, M Herbig, ... Clinical Immunology 208, 108232 | 2019 | |
| Morpho‐Rheological Fingerprinting of Rod Photoreceptors Using Real‐Time Deformability Cytometry T Santos‐Ferreira, M Herbig, O Otto, M Carido, MO Karl, S Michalakis, ... Cytometry Part A 95 (11), 1145-1157 | 2019 | |
| 3D microenvironment stiffness regulates tumor spheroid growth and mechanics via p21 and ROCK AV Taubenberger, S Girardo, N Träber, E Fischer‐Friedrich, M Kräter, ... Advanced Biosystems 3 (9), 1900128 | 2 | 2019 |
| Controlling distinct signaling states in cultured cancer cells provides a new platform for drug discovery SW Poser, O Otto, C Arps-Forker, Y Ge, M Herbig, C Andree, ... The FASEB Journal 33 (8), 9235-9249 | 1 | 2019 |
| Erratum: Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation. S Tietze, M Kräter, A Jacobi, A Taubenberger, M Herbig, R Wehner, ... Advanced science (Weinheim, Baden-Wurttemberg, Germany) 6 (8), 1900622-1900622 | 1* | 2019 |
| Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation S Tietze, M Kräter, A Jacobi, A Taubenberger, M Herbig, R Wehner, ... Advanced Science 6 (8), 1802104 | 1 | 2019 |
| Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry A Jacobi, P Rosendahl, M Kräter, M Urbanska, M Herbig, J Guck Stem Cell Mobilization, 135-148 | 2019 | |
| Buckling of epithelium growing under spherical confinement A Trushko, I Di Meglio, A Merzouki, C Blanch-Mercader, S Abuhattum, ... bioRxiv, 513119 | 2 | 2019 |
| Oncogenic signaling alters cell shape and mechanics to facilitate cell division under confinement. HK Matthews, S Ganguli, K Plak, AV Taubenberger, M Piel, J Guck, ... bioRxiv, 571885 | 2019 | |
| The Relationship Between Metastatic Potential and In Vitro Mechanical Properties of Osteosarcoma Cells CN Holenstein, A Horvath, B Schär, AD Schoenenberger, M Bollhalder, ... Molecular Biology of the Cell 30 (7), 887-898 | 2 | 2019 |
| Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming KR Bashant, A Vassallo, C Herold, R Berner, L Menschner, ... Journal of Leukocyte Biology, e12967 | 6 | 2019 |
| Droplet-Assisted Microfluidic Fabrication and Characterization of Multifunctional Polysaccharide Microgels Formed by Multicomponent Reactions N Hauck, N Seixas, S Centeno, R Schlüßler, G Cojoc, P Müller, J Guck, ... Polymers 10 (10), 1055 | 12 | 2018 |
| Mechanical mapping of spinal cord growth and repair in living zebrafish larvae by Brillouin imaging R Schlüßler, S Möllmert, S Abuhattum, G Cojoc, P Müller, K Kim, C Möckel, ... Biophysical journal 115 (5), 911-923 | 31 | 2018 |
| Comparative study of cell mechanics methods PH Wu, DRB Aroush, A Asnacios, WC Chen, ME Dokukin, BL Doss, ... Nature methods 15 (7), 491 | 2018 | |
| A comparison of methods to assess cell mechanical properties PH Wu, DRB Aroush, A Asnacios, WC Chen, ME Dokukin, BL Doss, ... Nature Methods 15 (7), 491-498 | 75 | 2018 |
| Response to Comment on “Cell nuclei have lower refractive index and mass density than cytoplasm” P Müller, J Guck Journal of Biophotonics 11 (6), e201800095 | 1 | 2018 |
| Real-time fluorescence and deformability cytometry P Rosendahl, K Plak, A Jacobi, M Kraeter, N Toepfner, O Otto, C Herold, ... Nature Methods 15 (5), 355-358 | 31 | 2018 |
| Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging P Müller, M Schürmann, S Girardo, G Cojoc, J Guck Optics Express 26 (8), 10729-10743 | 5 | 2018 |
| Toll-like receptor-mediated upregulation of CXCL16 in psoriasis orchestrates neutrophil activation S Steffen, S Abraham, M Herbig, F Schmidt, K Blau, S Meisterfeld, ... Journal of Investigative Dermatology 138 (2), 344-354 | 8 | 2018 |
| Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood N Toepfner, C Herold, O Otto, P Rosendahl, A Jacobi, M Kräter, ... eLife 7, e29213 | 33 | 2018 |
| High-throughput microfluidic characterization of erythrocyte shape and mechanical variability F Reichel, J Mauer, AA Nawaz, G Gompper, J Guck, DA Fedosov bioRxiv, 488189 | 2018 | |
| Alterations in Cell Mechanics by Actin Cytoskeletal Changes Correlate with Strain-Specific Rubella Virus Phenotypes for Cell Migration and Induction of Apoptosis M Kräter, J Sapudom, N Bilz, T Pompe, J Guck, C Claus Cells 7 (9), 136 | 2 | 2018 |
| Acute but not inherited demyelination in mouse models leads to brain tissue stiffness changes D Eberle, G Fodelianaki, T Kurth, A Jagielska, S Moellmert, E Ulbricht, ... bioRxiv, 449603 | 3 | 2018 |
| Axonal transport, phase-separated compartments and neuron mechanics—a new approach to investigate neurodegenerative diseases M Nötzel, G Rosso, S Möllmert, A Seifert, R Schlüßler, K Kim, A Hermann, ... Frontiers in Cellular Neuroscience 12, 358 | 2 | 2018 |
| High-throughput single-cell mechanical phenotyping with real-time deformability cytometry. M Urbanska, P Rosendahl, M Kräter, J Guck Methods in Cell Biology 147, 175-198 | 6 | 2018 |
| Metabolic profiling of human eosinophils L Porter, N Toepfner, KR Bashant, J Guck, M Ashcroft, N Farahi, ... Frontiers in Immunology 9, 1404 | 4 | 2018 |
| Standardized microgel beads as elastic cell mechanical probes S Girardo, N Traeber, K Wagner, G Cojoc, C Herold, R Goswami, ... Journal of Materials Chemistry B | 21 | 2018 |
| Intracellular mass density increase is accompanying but not sufficient for stiffening and growth arrest of yeast cells S Abuhattum, K Kim, TM Franzmann, A Esslinger, D Midtvedt, ... Frontiers in Physics 6, 39-50 | 3 | 2018 |
| Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells M Herbig, M Kräter, K Plak, P Müller, J Guck, O Otto Flow Cytometry Protocols – Methods in Molecular Biology 1678, 347-369 | 19 | 2018 |
| Mechanical mapping of spinal cord development and repair in living zebrafish larvae using Brillouin microscopy R Schlüßler, S Möllmert, S Abuhattum, G Cojoc, P Müller, K Kim, C Möckel, ... bioRxiv, 181560 | 3 | 2018 |
| Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage M Urbanska, M Winzi, K Neumann, S Abuhattum, P Rosendahl, P Müller, ... Development 144 (23), 4313-4321 | 4 | 2017 |
| Three-dimensional correlative single‐cell imaging utilizing fluorescence and refractive index tomography M Schürmann, G Cojoc, S Girardo, E Ulbricht, J Guck, P Müller Journal of Biophotonics 81 (2), e201700145 | 36 | 2017 |
| Method for diagnosis and/or prognosis of cancers by analysis of the mechanical properties of tumor cells J Käs, J Guck US Patent 9,683,982 | 2017 | |
| 3D single-cell imaging using combined fluorescence and refractive index tomography M Schürmann, G Cojoc, S Girardo, E Ulbricht, J Guck, P Müller arXiv preprint arXiv:1706.00715 | 2017 | |
| Mechanical deformation induces depolarization of neutrophils AE Ekpenyong, N Toepfner, C Fiddler, M Herbig, W Li, G Cojoc, ... Science Advances 3 (6), e1602536 | 24 | 2017 |
| Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells S Tavares, AF Vieira, AV Taubenberger, M Araújo, NP Martins, ... Nature Communications 8, 15237 | 45 | 2017 |
| Mechanical strain promotes oligodendrocyte differentiation by global changes of gene expression A Jagielska, AL Lowe, E Makhija, L Wroblewska, J Guck, RJM Franklin, ... Frontiers in cellular neuroscience 11, 93 | 25 | 2017 |
| Initiation of acute graft-versus-host disease by angiogenesis K Riesner, Y Shi, A Jacobi, M Kraeter, M Kalupa, A McGearey, S Mertlitz, ... Blood 129 (14), 2021-2032 | 23 | 2017 |
| Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion M Koch, KE Wright, O Otto, M Herbig, ND Salinas, NH Tolia, TJ Satchwell, ... Proceedings of the National Academy of Sciences 114 (16), 4225-4230 | 26 | 2017 |
| High‐throughput cell mechanical phenotyping for label‐free titration assays of cytoskeletal modifications S Golfier, P Rosendahl, A Mietke, M Herbig, J Guck, O Otto Cytoskeleton 74 (8), 283-296 | 21 | 2017 |
| Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase CJ Chan, W Li, G Cojoc, J Guck Biophysical Journal 112 (6), 1063-1076 | 10 | 2017 |
| V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling-a new option for HCC therapy K Bartel, M Winzi, M Ulrich, A Koeberle, D Menche, O Werz, R Müller, ... Oncotarget 8 (6), 9476 | 17 | 2017 |
| Numerical simulation of real-time deformability cytometry to extract cell mechanical properties M Mokbel, D Mokbel, A Mietke, N Traber, S Girardo, O Otto, J Guck, ... ACS Biomaterials Science & Engineering 3 (11), 2962-2973 | 47 | 2017 |
| Real-time fluorescence and deformability cytometry-flow cytometry goes mechanics P Rosendahl, K Plak, A Jacobi, M Kraeter, N Toepfner, O Otto, C Herold, ... bioRxiv, 187435 | 2 | 2017 |
| Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion A Surcel, ES Schiffhauer, D Thomas, Q Zhu, K DiNapoli, M Herbig, O Otto, ... bioRxiv, 190553 | 5 | 2017 |
| Roadmap on optofluidics P Minzioni, R Osellame, C Sada, S Zhao, F Omenetto, K Gylfason, ... Journal of Optics 19 (9), 093003 | 30 | 2017 |
| Bone marrow niche-mimetics modulate HSPC function via integrin signaling M Kräter, A Jacobi, O Otto, S Tietze, K Müller, DM Poitz, S Palm, VM Zinna, ... Scientific Reports 7 (1), 2549 | 12 | 2017 |
| Detection Of Human Disease Conditions By Single-Cell Morpho-Rheological Phenotyping Of Whole Blood N Toepfner, C Herold, O Otto, P Rosendahl, A Jacobi, M Krater, ... bioRxiv, 145078 | 3 | 2017 |
| Mechanical mismatch between Ras transformed and untransformed epithelial cells C Gullekson, G Cojoc, M Schürmann, J Guck, A Pelling Soft Matter 13 (45), 8483-8491 | 3 | 2017 |
| Enlightening discriminative network functional modules behind Principal Component Analysis separation in differential-omic science studies S Ciucci, Y Ge, C Durán, A Palladini, V Jiménez-Jiménez, ... Scientific Reports 7, 43946 | 26 | 2017 |
| Niche WNT5A regulates the actin cytoskeleton during regeneration of hematopoietic stem cells C Schreck, R Istvánffy, C Ziegenhain, T Sippenauer, F Ruf, L Henkel, ... Journal of Experimental Medicine 214 (1), 156-181 | 13 | 2016 |
| Initiation of Acute Graft-Versus-Host Disease By Angiogenesis K Riesner, Y Shi, A Jacobi, M Kraeter, M Kalupa, A McGearey, S Mertlitz, ... Blood, The Journal of the American Society of Hematology 128 (22), 4533-4533 | 2016 | |
| Chemotherapy impedes in vitro microcirculation and promotes migration of leukemic cells with impact on metastasis SV Prathivadhi-Bhayankaram, J Ning, M Mimlitz, C Taylor, E Gross, ... Biochemical and Biophysical Research Communications 479 (4), 841-846 | 5 | 2016 |
| Brain tissue stiffness is a sensitive marker for acidosis K Holtzmann, HOB Gautier, AF Christ, J Guck, RT Káradóttir, K Franze Journal of Neuroscience Methods 271, 50-54 | 17 | 2016 |
| The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512 H Mziaut, B Mulligan, P Hoboth, O Otto, A Ivanova, M Herbig, ... Molecular Metabolism 5 (8), 656-668 | 11 | 2016 |
| Apparatus and method for determining the mechanical properties of cells J Guck, O Otto, P Rosendahl US Patent App. 14/913,699 | 2 | 2016 |
| A nanoprinted model of interstitial cancer migration reveals a link between cell deformability and proliferation M Panagiotakopoulou, M Bergert, A Taubenberger, J Guck, D Poulikakos, ... ACS Na0 (7), 6437-6448 | 29 | 2016 |
| Real-time deformability cytometry (RT-DC) as novel diagnostic tool in myelodysplastic syndromes E Bulycheva, O Otto, M Herbig, H Weidner, U Baschant, S Herold, ... Haematologica 101, 494-495 | 2016 | |
| 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments AV Taubenberger, LJ Bray, B Haller, A Shaposhnykov, M Binner, ... Acta Biomaterialia 36, 73-85 | 63 | 2016 |
| Mechanical phenotyping of leukemic blast cells using real-time deformability cytometry J Stächele, N Töpfner, C Herold, R Berner, M Bornhäuser, J Guck, ... Klinische Pädiatrie 228 (03), A2 | 2016 | |
| Cell nuclei have lower refractive index and mass density than cytoplasm M Schürmann, J Scholze, P Müller, J Guck, CJ Chan Journal of Biophotonics 9 (10), 1068-1076 | 77 | 2016 |
| A pH-driven transition of the cytoplasm from a fluid-to a solid-like state promotes entry into dormancy MC Munder, D Midtvedt, T Franzmann, E Nu?ske, O Otto, M Herbig, ... eLife 5, e09347 | 140 | 2016 |
| Mechanosensing is critical for axon growth in the developing brain DE Koser, AJ Thompson, SK Foster, A Dwivedy, GK Sheridan, E Pillai, ... Nature Neuroscience 19 (12), 1592-1598 | 174 | 2016 |
| Materials and technologies for soft implantable neuroprostheses S Lacour, G Courtine, J Guck Nature Reviews Materials 1, 16063 | 162 | 2016 |
| Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry M Xavier, P Rosendahl, M Herbig, M Kräter, D Spencer, M Bornhäuser, ... Integrative Biology 8 (5), 616-623 | 31 | 2016 |
| Refractive index measurements of single, spherical cells using digital holographic microscopy M Schürmann, J Scholze, P Müller, CJ Chan, AE Ekpenyong, KJ Chalut, ... Methods in cell biology 125, 143-159 | 27 | 2015 |
| ODTbrain: a Python library for full-view, dense diffraction tomography P Müller, M Schürmann, J Guck BMC bioinformatics 16 (1), 367 | 18 | 2015 |
| Mechanotransduction in neutrophil activation and deactivation AE Ekpenyong, N Toepfner, ER Chilvers, J Guck Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1853 (11), 3105-3116 | 22 | 2015 |
| Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment A Mietke, O Otto, S Girardo, P Rosendahl, A Taubenberger, S Golfier, ... Biophysical Journal 109 (10), 2023-2036 | 91 | 2015 |
| ODTbrain: a Python library for full-view, dense diffraction tomography P Müller, M Schürmann, J Guck BMC Bioinformatics 16 (1), 367 | 18 | 2015 |
| Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia. M Wobus, M Bornhäuser, A Jacobi, M Kräter, O Otto, C Ortlepp, J Guck, ... Oncotarget 6 (36), 38804-38815 | 12 | 2015 |
| Single-cell diffraction tomography with optofluidic rotation about a tilted axis P Müller, M Schürmann, CJ Chan, J Guck Optical Trapping and Optical Micromanipulation XII 9548, 95480U | 11 | 2015 |
| The Theory of Diffraction Tomography P Müller, M Schürmann, J Guck arXiv preprint arXiv:1507.00466 | 31 | 2015 |
| Myosin II Activity Softens Cells in Suspension CJ Chan, AE Ekpenyong, S Golfier, W Li, KJ Chalut, O Otto, J Elgeti, ... Biophysical Journal 108 (8), 1856-1869 | 64 | 2015 |
| Real-time deformability cytometry: on-the-fly cell mechanical phenotyping O Otto, P Rosendahl, A Mietke, S Golfier, C Herold, D Klaue, S Girardo, ... Nature Methods 12 (3), 199–202 | 315 | 2015 |
| Deformation of phospholipid vesicles in an optical stretcher U Delabre, K Feld, E Crespo, G Whyte, C Sykes, U Seifert, J Guck Soft Matter 11 (30), 6075-6088 | 26 | 2015 |
| A monolithic glass chip for active single-cell sorting based on mechanical phenotyping C Faigle, F Lautenschläger, G Whyte, P Homewood, E Martín-Badosa, ... Lab on a Chip 15 (5), 1267-1275 | 26 | 2015 |
| Actin polymerization as a key innate immune effector mechanism to control Salmonella infection SM Man, A Ekpenyong, P Tourlomousis, S Achouri, E Cammarota, ... Proceedings of the National Academy of Sciences 111 (49), 17588-17593 | 61 | 2014 |
| Separation of blood cells with differing deformability using deterministic lateral displacement D Holmes, G Whyte, J Bailey, N Vergara-Irigaray, A Ekpenyong, J Guck, ... Interface focus 4 (6), 20140011 | 73 | 2014 |
| Dynamic operation of optical fibres beyond the single-mode regime facilitates the orientation of biological cells M Kreysing, D Ott, MJ Schmidberger, O Otto, M Schürmann, ... Nature Communications 5, 5481-5486 | 33 | 2014 |
| Direct observation of light focusing by single photoreceptor cell nuclei Z B?aszczak, M Kreysing, J Guck Optics Express 22 (9), 11043-11060 | 15 | 2014 |
| The relationship between glial cell mechanosensitivity and foreign body reactions in the central nervous system P Moshayedi, G Ng, JCF Kwok, GSH Yeo, CE Bryant, JW Fawcett, ... Biomaterials 35 (13), 3919-3925 | 155 | 2014 |
| Impact of heating on passive and active biomechanics of suspended cells CJ Chan, G Whyte, L Boyde, G Salbreux, J Guck Interface focus 4 (2), 20130069 | 25 | 2014 |
| Grouped retinae and tapetal cups in some Teleostian fish: Occurrence, structure, and function M Francke, M Kreysing, A Mack, J Engelmann, A Karl, F Makarov, J Guck, ... Progress in Retinal and Eye Research 38, 43-69 | 17 | 2014 |
| SAMHD1 prevents autoimmunity by maintaining genome stability S Kretschmer, C Wolf, N König, W Staroske, J Guck, M Häusler, H Luksch, ... Annals of the rheumatic diseases 74 (3), e17-e17 | 101 | 2014 |
| Method for diagnosis and/or prognosis of cancers by analysis of the mechanical properties of tumour cells J Käs, J Guck US Patent App. 13/877,452 | 2013 | |
| Mechanics Meets Medicine J Guck, ER Chilvers Science translational medicine 5 (212), 212fs41-212fs41 | 32 | 2013 |
| Mechanics in neuronal development and repair K Franze, PA Janmey, J Guck Annual review of biomedical engineering 15, 227-251 | 166 | 2013 |
| Bacterial infection of macrophages induces decrease in refractive index AE Ekpenyong, SM Man, S Achouri, CE Bryant, J Guck, KJ Chalut Journal of biophotonics 6 (5), 393-397 | 37 | 2013 |
| Elastic theory for the deformation of a solid or layered spheroid under axisymmetric loading L Boyde, A Ekpenyong, G Whyte, J Guck Acta Mechanica 224 (4), 819-839 | 7 | 2013 |
| Cell Sorting J Guck Handbook of Biophotonics, 143-149 | 2013 | |
| Chromatin decondensation and nuclear softening accompany Nanog downregulation in embryonic stem cells KJ Chalut, M Höpfler, F Lautenschläger, L Boyde, CJ Chan, A Ekpenyong, ... Biophysical Journal 103 (10), 2060-2070 | 108 | 2012 |
| Comparison of stresses on homogeneous spheroids in the optical stretcher computed with geometrical optics and generalized Lorenz–Mie theory L Boyde, A Ekpenyong, G Whyte, J Guck Applied optics 51 (33), 7934-7944 | 23 | 2012 |
| Femtosecond laser fabricated monolithic devices for single cell manipulation F Bragheri, P Minzioni, RM Vazquez, N Bellini, P Paiè, C Mondello, ... Frontiers in Optics, FW1G. 2 | 1 | 2012 |
| Validation and perspectives of a femtosecond laser fabricated monolithic optical stretcher N Bellini, F Bragheri, I Cristiani, J Guck, R Osellame, G Whyte Biomedical Optics Express 3 (10), 2658-2668 | 39 | 2012 |
| Viscoelastic properties of differentiating blood cells are fate-and function-dependent AE Ekpenyong, G Whyte, K Chalut, S Pagliara, F Lautenschläger, ... PloS one 7 (9), e45237 | 123 | 2012 |
| Changes in Ect2 localization couple actomyosin-dependent cell shape changes to mitotic progression HK Matthews, U Delabre, JL Rohn, J Guck, P Kunda, B Baum Developmental Cell 23 (2), 371-383 | 139 | 2012 |
| Coupling of active motion and advection shapes intracellular cargo transport PK Trong, J Guck, RE Goldstein Physical Review Letters 109 (2), 028104 | 26 | 2012 |
| Photonic crystal light collectors in fish retina improve vision in turbid water M Kreysing, R Pusch, D Haverkate, M Landsberger, J Engelmann, ... Science 336 (6089), 1700-1703 | 55 | 2012 |
| Mechanical environment modulates biological properties of oligodendrocyte progenitor cells A Jagielska, AL Norman, G Whyte, KJV Vliet, J Guck, RJM Franklin Stem cells and development 21 (16), 2905-2914 | 75 | 2012 |
| Live cells as optical fibers in the vertebrate retina A Reichenbach, K Franze, S Agte, S Junek, A Wurm, J Grosche, ... Selected topics on optical fiber technology 10, 247-270 | 13 | 2012 |
| Lebendige Optik J Guck Physik Journal 11 (6) | 2012 | |
| Quantifying cellular differentiation by physical phenotype using digital holographic microscopy KJ Chalut, AE Ekpenyong, WL Clegg, IC Melhuish, J Guck Integrative Biology 4 (3), 280-284 | 65 | 2012 |
| Device and method for the contactless manipulation and alignment of sample particles in a measurement volume using a nonhomogeneous electric alternating field M Kreysing, J Guck, J Kaes US Patent 8,076,632 | 15 | 2011 |
| Exact analytical expansion of an off-axis Gaussian laser beam using the translation theorems for the vector spherical harmonics L Boyde, KJ Chalut, J Guck Applied optics 50 (7), 1023-1033 | 9 | 2011 |
| Near-and far-field scattering from arbitrary three-dimensional aggregates of coated spheres using parallel computing L Boyde, KJ Chalut, J Guck Physical Review E 83 (2), 026701 | 23 | 2011 |
| 3D inverted colloidal crystals in realistic cell migration assays for drug screening applications J da Silva, F Lautenschläger, CHR Kuo, J Guck, E Sivaniah Integrative Biology 3 (12), 1202-1206 | 14 | 2011 |
| Spatial mapping of the mechanical properties of the living retina using scanning force microscopy K Franze, M Francke, K Günter, AF Christ, N Körber, A Reichenbach, ... Soft Matter 7 (7), 3147-3154 | 75 | 2011 |
| Mechanical difference between white and gray matter in the rat cerebellum measured by scanning force microscopy AF Christ, K Franze, H Gautier, P Moshayedi, J Fawcett, RJM Franklin, ... Journal of Biomechanics 43 (15), 2986-2992 | 177 | 2010 |
| Mesenchymal stem cell mechanics from the attached to the suspended state JM Maloney, D Nikova, F Lautenschläger, E Clarke, R Langer, J Guck, ... Biophysical Journal 99 (8), 2479-2487 | 131 | 2010 |
| Micro and nanotechnology for biological and biomedical applications CT Lim, J Han, J Guck, H Espinosa Medical & biological engineering & computing 48 (10), 941-943 | 32 | 2010 |
| Biophotonic techniques for the study of malaria-infected red blood cells JMA Mauritz, A Esposito, T Tiffert, JN Skepper, A Warley, YZ Yoon, ... Medical & biological engineering & computing 48 (10), 1055-1063 | 28 | 2010 |
| The biophysics of neuronal growth K Franze, J Guck Reports on Progress in Physics 73 (9), 094601 | 124 | 2010 |
| Dual-beam laser traps in biology and medicine: when one beam is not enough G Whyte, F Lautenschläger, M Kreysing, L Boyde, A Ekpenyong, ... Optical Trapping and Optical Micromanipulation VII 7762, 77620G | 2 | 2010 |
| Physical insight into light scattering by photoreceptor cell nuclei M Kreysing, L Boyde, J Guck, KJ Chalut Optics Letters 35 (15), 2639-2641 | 34 | 2010 |
| Monitoring of laser micromanipulated optically trapped cells by digital holographic microscopy B Kemper, P Langehanenberg, A Höink, G von Bally, F Wottowah, ... Journal of biophotonics 3 (7), 425-431 | 17 | 2010 |
| Mechanosensitivity of astrocytes on optimized polyacrylamide gels analyzed by quantitative morphometry P Moshayedi, L da F Costa, A Christ, SP Lacour, J Fawcett, J Guck, ... Journal of Physics: Condensed Matter 22 (19), 194114 | 96 | 2010 |
| Detection of Plasmodium falciparum-infected red blood cells by optical stretching JMA Mauritz, T Tiffert, R Seear, F Lautenschläger, A Esposito, VL Lew, ... Journal of biomedical optics 15 (3), 030517-030517-3 | 39 | 2010 |
| The cavity-to-cavity migration of leukaemic cells through 3D honey-combed hydrogels with adjustable internal dimension and stiffness J da Silva, F Lautenschläger, E Sivaniah, JR Guck Biomaterials 31 (8), 2201-2208 | 47 | 2010 |
| Critical review: cellular mechanobiology and amoeboid migration J Guck, F Lautenschläger, S Paschke, M Beil Integrative Biology 2 (11-12), 575-583 | 84 | 2010 |
| Microfluidic integration of high power dual-beam laser traps for cell mechanical measurements F Lautenschlaeger, JR Guck 2009 International Symposium on Optomechatronic Technologies, 419-422 | 3 | 2009 |
| The regulatory role of cell mechanics for migration of differentiating myeloid cells F Lautenschläger, S Paschke, S Schinkinger, A Bruel, M Beil, J Guck Proceedings of the National Academy of Sciences 106 (37), 15696-15701 | 198 | 2009 |
| Interaction of Gaussian beam with near-spherical particle: an analytic-numerical approach for assessing scattering and stresses L Boyde, KJ Chalut, J Guck JOSA A 26 (8), 1814-1826 | 37 | 2009 |
| Do cells care about physics? J Guck Physics World 22 (7), 31-34 | 3 | 2009 |
| Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution I Solovei, M Kreysing, C Lanctôt, S Kösem, L Peichl, T Cremer, J Guck, ... Cell 137 (2), 356-368 | 586 | 2009 |
| Oral cancer diagnosis by mechanical phenotyping TW Remmerbach, F Wottawah, J Dietrich, B Lincoln, C Wittekind, J Guck Cancer Research 69 (5), 1728-1732 | 280 | 2009 |
| Corrigendum to “Quantifying the contribution of actin networks to the elastic strength of fibroblasts”[J. Theor. Biol. 242 (2006) 502–516] R Ananthakrishnan, J Guck, F Wottawah, S Schinkinger, B Lincoln, ... Journal of Theoretical Biology 255 (1), 162 | 1 | 2008 |
| The optical cell rotator MK Kreysing, T Kießling, A Fritsch, C Dietrich, JR Guck, JA Käs Optics Express 16 (21), 16984-16992 | 112 | 2008 |
| High‐Throughput Rheological Measurements with an Optical Stretcher B Lincoln, F Wottawah, S Schinkinger, S Ebert, J Guck Methods in cell biology 83, 397-423 | 73 | 2007 |
| Fluorescence ratio thermometry in a microfluidic dual-beam laser trap S Ebert, K Travis, B Lincoln, J Guck Optics Express 15 (23), 15493-15499 | 105 | 2007 |
| Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications B Lincoln, S Schinkinger, K Travis, F Wottawah, S Ebert, F Sauer, J Guck Biomedical Microdevices 9 (5), 703-710 | 137 | 2007 |
| Müller cells are living optical fibers in the vertebrate retina K Franze, J Grosche, SN Skatchkov, S Schinkinger, C Foja, D Schild, ... Proceedings of the National Academy of Sciences 104 (20), 8287-8292 | 355 | 2007 |
| Viscoelastic properties of individual glial cells and neurons in the CNS YB Lu, K Franze, G Seifert, C Steinhäuser, F Kirchhoff, H Wolburg, J Guck, ... Proceedings of the National Academy of Sciences 103 (47), 17759-17764 | 449 | 2006 |
| Quantifying the contribution of actin networks to the elastic strength of fibroblasts R Ananthakrishnan, J Guck, F Wottawah, S Schinkinger, B Lincoln, ... Journal of theoretical biology 242 (2), 502-516 | 99 | 2006 |
| Scattering from Single Nanoparticles: Mie theory revisited K Travis, J Guck Biophysical Reviews and Letters 1 (02), 179-207 | 2 | 2006 |
| The microscopy cell (MicCell), a versatile modular flowthrough system for cell biology, biomaterial research, and nanotechnology FU Gast, PS Dittrich, P Schwille, M Weigel, M Mertig, J Opitz, U Queitsch, ... Microfluidics and Nanofluidics 2 (1), 21-36 | 70 | 2006 |
| Excitation beyond the monochromatic laser limit: simultaneous 3-D confocal and multiphoton microscopy with a tapered fiber as white-light laser source T Betz, J Teipel, D Koch, W Härtig, J Guck, J Käs, H Giessen Journal of biomedical optics 10 (5), 054009-054009-7 | 26 | 2005 |
| Characterizing single suspended cells by optorheology F Wottawah, S Schinkinger, B Lincoln, S Ebert, K Müller, F Sauer, K Travis, ... Acta biomaterialia 1 (3), 263-271 | 50 | 2005 |
| Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence J Guck, S Schinkinger, B Lincoln, F Wottawah, S Ebert, M Romeyke, ... Biophysical Journal 88 (5), 3689-3698 | 1179 | 2005 |
| Modelling the structural response of an eukaryotic cell in the optical stretcher R Ananthakrishnan, J Guck, F Wottawah, S Schinkinger, B Lincoln, ... Current Science 88 (9) | 24 | 2005 |
| Optical rheology of biological cells F Wottawah, S Schinkinger, B Lincoln, R Ananthakrishnan, M Romeyke, ... Physical review letters 94 (9), 098103 | 235 | 2005 |
| Feeling for cells with light S Schinkinger, F Wottawah, K Travis, B Lincoln, J Guck Optical Trapping and Optical Micromanipulation 5514, 170-178 | 4 | 2004 |
| Deformability‐based flow cytometry B Lincoln, HM Erickson, S Schinkinger, F Wottawah, D Mitchell, S Ulvick, ... Cytometry Part A 59 (2), 203-209 | 179 | 2004 |
| Stretching biological cells with light J Guck, R Ananthakrishnan, CC Cunningham, J Käs Journal of Physics: Condensed Matter 14 (19), 4843 | 102 | 2002 |
| The optical stretcher: a novel laser tool to micromanipulate cells J Guck, R Ananthakrishnan, H Mahmood, TJ Moon, CC Cunningham, ... Biophysical Journal 81 (2), 767-784 | 922 | 2001 |
| Optical deformability: micromechanics from cell research to biomedicine JR Guck PhD Thesis | 6 | 2001 |
| Optical deformability of soft biological dielectrics J Guck, R Ananthakrishnan, TJ Moon, CC Cunningham, J Käs Physical Review Letters 84 (23), 5451 | 330 | 2000 |
| Optical stretcher JA Kas, JR Guck US Patent 6,067,859 | 63 | 2000 |
| Dynamics of Single Protein Polymers Visualized by Fluorescence Microscopy J Käs, J Guck, D Humphrey Modern Optics, Electronics and High Precision Techniques in Cell Biology … | 1 | 1998 |
| Optical Stretching–automatisierte Zell-Sortierung und Charakterisierung S Schinkinger, F Wottawah, B Lincoln, J Guck LABORWELT, 4 |
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