Neuro Probe AP48 chemotaxis chamber可重復(fù)利用的MB系列趨化小室產(chǎn)品可以用于趨化實(shí)驗(yàn)，腫瘤細(xì)胞的侵襲實(shí)驗(yàn)及細(xì)胞與細(xì)胞的相互作用的研究。設(shè)計(jì)上也是采用96微孔標(biāo)準(zhǔn)板，可與自動(dòng)工作站兼容，并且下室采用可以移動(dòng)的方式，很方便非貼壁的細(xì)胞實(shí)驗(yàn)。同時(shí)，MB系列可以兼容其它系列的趨化板，也適合貼壁細(xì)胞的培養(yǎng)。造型上MB系列的板是上下兩塊板通過螺絲可以緊密連接在一起，上面的板子

Neuro Probe AP48 chemotaxis chamber可重復(fù)利用的MB系列趨化小室產(chǎn)品可以用于趨化實(shí)驗(yàn)，腫瘤細(xì)胞的侵襲實(shí)驗(yàn)及細(xì)胞與細(xì)胞的相互作用的研究。設(shè)計(jì)上也是采用96微孔標(biāo)準(zhǔn)板，可與自動(dòng)工作站兼容，并且下室采用可以移動(dòng)的方式，很方便非貼壁的細(xì)胞實(shí)驗(yàn)。同時(shí)，MB系列可以兼容其它系列的趨化板，也適合貼壁細(xì)胞的培養(yǎng)。造型上MB系列的板是上下兩塊板通過螺絲可以緊密連接在一起，上面的板子是96孔板的上室，下部板是一個(gè)大的凹槽支撐著微孔板形成下室。濾膜被放置在微孔板上，可以對(duì)微孔板進(jìn)行熒光檢測(cè)，細(xì)胞密度檢測(cè)和流式檢測(cè)來統(tǒng)計(jì)遷移細(xì)胞的的數(shù)量。

產(chǎn)品詳細(xì)信息

可重復(fù)利用的MB系列趨化小室產(chǎn)品可以用于趨化實(shí)驗(yàn)，腫瘤細(xì)胞的侵襲實(shí)驗(yàn)及細(xì)胞與細(xì)胞的相互作用的研究。

 

貨號(hào)	面積（mm2）	上室體積（ul）	下室體積（ul）	濾膜規(guī)格
AP48	8mm2	50 ul	25ul	25 x 80mm 聚碳酸脂膜 25 x 80mm 硝酸纖維素膜

 

In the Neuro Probe AP48 chemotaxis chamber, chemoattractants added to the lower wells act on cells in a suspension added to the upper wells. A filter separates the upper and lower wells. After incubation the migrated adherent cells on the underside of the filter are stained and counted.

The AP48 consists of precision-machined acrylic top and bottom plates, assembly hardware, and a silicone gasket. The bottom plate has 48 wells, each with 25µL volume and a diameter of 3.2mm. Corresponding holes in the top plate form the upper wells when the chamber is assembled. A single 25 x 80mm piece of filter membrane is placed between the top and bottom plates, and a gasket is positioned over the filter to create a seal. Narrow raised rims around mating surfaces of top and bottom wells enhance the seal.

Before the migrated cells can be stained and counted, the nonmigrated cells must be wiped off the top side of the filter. An accessory pack (stock # P48AP)includes instruments for this purpose.

The AP48 is designed for use with polycarbonate and cellulose nitrate filters. Please note that we also sell the AC48, a 48-well chamber specially designed for use with cellulose nitrate filters

Accompanying each chamber is an AP48 protocol for its use and care.

	Stock #	Exposed filter area	Bottom well volume	Top well volume	Filters used
	AP48	8mm2	25µL	50µL	25 x 80mm polycarbonate 25 x 80mm cellulose nitrate

AP48 Kit

Description

The AP48 Kit is a convenient, cost effective combination of the most popular items in our line of 48-well reusable chemotaxis instruments, filters, and accessories. You provide the cells, reagents, and stain.

The Kit includes:

•  (1) AP48 microchemotaxis chamber,
•  (1) P48AP accessory pack for handling polycarbonate filters, including:
  • (1) filter wiper for mounting on a ring stand
  • (1) large plastic filter clamp,
  • (1) small stainless steel filter clamp
  • (2) curved stainless steel forceps
  • Filter options —  A: (100 ) pieces of 3µm, 5µm or 8µm,  25x80mm filters; or B: (50) pieces each of 2 of the 3 pore sizes (3µm, 5µm and 8µm). Please enter your filter option in the Order Notes field on the Checkout screen.

NOTE – the AP48 is designed for use with adherent cells that will be counted visually on the bottom of the filter after migration. If you will be using non-adherent cells, or wish to count cells with a microplate reader, please see the ChemoTx® system or the MB-series 96-well chambers, or contact us for assistance.