產(chǎn)品描述

一瓶封閉緩沖液，500毫升。所有western印跡只需要5分鐘的封閉

每個(gè)印跡-5分鐘每個(gè)印跡阻斷

五分鐘的阻斷緩沖液，適用于所有印跡。

EveryBlot阻斷緩沖液為所有蛋白質(zhì)印跡提供5分鐘的阻斷和較大靈敏度，無論檢測方法如何。

Bio-rad伯樂封閉液試劑 12010020

功能和優(yōu)點(diǎn)

減少非特異性抗體結(jié)合以減少背景，同時(shí)保持優(yōu)異的敏感性

用于化學(xué)發(fā)光和熒光檢測

不含磷酸鹽緩沖液。非常適合與磷酸特異性抗體一起使用

5分鐘內(nèi)完成封閉

Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots

competitor-everyblot-5 minute everyblot blocking

A five minute blocking buffer for ALL western blots.

EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.

Features and Benefits

Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity

For both chemiluminescent and fluorescent detection

Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies

Complete blocking in 5 minutes

Bio-rad伯樂封閉液試劑 12010020

使用建議

   對于小型印跡，使用至少10 ml用于阻斷和抗體孵育步驟。對于中等大小的印跡，至少使用20毫升。

將塊加入膜中，攪拌孵育5分鐘

將第一抗體和第二抗體稀釋在全強(qiáng)度塊中，并在攪拌下孵育1小時(shí)

對于PVDF的熒光檢測，將SDS添加到第二抗體溶液中的0.02%。

   Recommended Protocol

For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.

Add block to the membrane and incubate for 5 minutes with agitation

Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation

For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.