Hu人結(jié)核菌桿抗體IgG(TB-AbIgG)北京

Mo小鼠催產(chǎn)素(OT)

COMPETITION ELISAThe ELISA protocols for detection of the antibody binding to an antigen-coated microtitre plate are standard laboratory techniques and will not be described here. We will just mention that most recombinant antibody fragments are typically detected using monoclonal antibodies directed against a peptidic tag engineered at the C-terminal extremity of the recombinant antibody. In certain experimental conditions, such peptidic tags may undergo proteolytic cleavage, thereby lowering the sensitivity of antibody detection. Reagents that bind to the antibody molecule without impairing antigen binding (e.g., protein A or protein L) may therefore be preferable. Alternatively, the experimental scheme described below can be performed in a similar fashion, using radiolabeled antibodies and radioacitve detection of antibody-mediated antigen binding. The concentration of purified antibody preparations is typically determined spectrophotometrically (1 mg/ml antibody solution absorbs 1.4 absorption units at 280 nm). If necessary (for example when using supernatants), the concentration of active antibody can be detected with a straightforward ELISA adaptation of the protocol mentioned above for the determination of antibody concentration by band-shift assay.

2.2.6 捕獲包被法測(cè)抗體IgM抗體的檢測(cè)用于傳染病的早期診斷中。間接法ELISA一般僅適用于檢測(cè)總抗體或IgG抗體。如用抗原包被的間接法直接測(cè)定IgM抗體，因標(biāo)本中一般同時(shí)存在較高濃度的IgG抗體，后者將競(jìng)爭(zhēng)結(jié)合固相抗原而使一部份IgM抗體不能結(jié)合到固相上。因此如用抗人IgM作為二抗，間接測(cè)定IgM抗體，必須先將標(biāo)本用A蛋白或抗IgG抗體處理，以除去IgG的干擾。在臨床檢驗(yàn)中測(cè)定抗體IgM時(shí)多采用捕獲包被法。先用抗人IgM抗體包被固相，以捕獲血清標(biāo)本中的IgM（其中包括針對(duì)抗原的特異性IgM抗體和非特異性的IgM）。然后加入抗原，此抗原僅與特異性IgM相結(jié)合。繼而加酶標(biāo)記針對(duì)抗原的特異性抗體。再與底物作用，呈色即與標(biāo)本中的IgM成正相關(guān)。此法常用于病毒性感染的早期診斷。甲型肝炎病毒（HAV）抗體的檢測(cè)模式見圖2-7。類風(fēng)濕因子（RF）同樣能干擾捕獲包被法測(cè)定IgM抗體，導(dǎo)致假陽(yáng)性反應(yīng)。因此中和IgG的間接法近來(lái)頗受青睞，用這類試劑檢測(cè)抗CMV IgGM和抗弓形蟲IgM抗體已獲成功。

Mo小鼠纖連蛋白(FN)

植物維生素D2(VD2)

大鼠生長(zhǎng)激素(GH)

魚總超氧化物歧化酶（SOD）

Human tumor specific antigen,TSA ELISA Kit

Hu人葡萄糖6磷酸異構(gòu)酶(GPI)

Rabbit anti-glycoprotein antibody,GP ELISA Kit

Mo小鼠凋亡誘導(dǎo)因子(AIF)

兔阿霉素(ADR)

大鼠內(nèi)du素(ET)

Rat Troponin T,Tn-T ELISA Kit

雞卵黃免疫球蛋白(IgY)

Hu人組蛋白H2b(histon-H2b)

Human Chorionic Gonadotrophin β,β-CG ELISA Kit

5.1 基本概念5.1.1 質(zhì)量控制（Quaility Control,Q.C）　　質(zhì)量控制是監(jiān)視全過(guò)程，排除誤差，防止變化，維持標(biāo)準(zhǔn)化現(xiàn)狀的一個(gè)管理過(guò)程。這一過(guò)程是通過(guò)一個(gè)反饋環(huán)路進(jìn)行的?！　?）確定控制的對(duì)象?！　?）規(guī)定控制對(duì)象的標(biāo)準(zhǔn)（預(yù)期值）?！　?）制定或選擇控制方法和手段?！　?）測(cè)量實(shí)際數(shù)據(jù)。