目錄:愛必信（上海）生物科技有限公司>>細(xì)胞生物學(xué)>>重組蛋白>> abs00912Recombinant Human IFN-α1b
| 供貨周期 | 一周 | 規(guī)格 | 2ug |
|---|
| 概述 | |
|---|---|
| 種屬 | Human |
| 形態(tài) | Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4, containing 4 % mannitol and 1 % HSA. |
| 來源 | Escherichia coli. |
| 氨基酸序列 | MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIIFNLFT TKDSSAAWDE DLLDKFC YLNDLEAC VMQEERVGET PLMNVDSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE |
| 內(nèi)毒素水平 | Less than 1 EU/µg of Recombinant HumanIFN-α1b as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 × 108 IU/mg. |
| Accession # | P01562 |
| 預(yù)期分子量 | Approximay 19.5 kDa, a single non-glycosylated polypeptide chain containing 167 amino acids. |
| 性能 | |
|---|---|
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 純度 | > 96 % by SDS-PAGE and HPLC analyses. |
| Gene IDs | 3439 |
| 參考文獻(xiàn) | |
|---|---|
| 1. Tarhini AA, Gogas H, Kirkwood JM. 2012. J Immunol, 189: 3789-93.<br/>2. Tohyama M, Yang L, Hanakawa Y, et al. 2012. J Invest Dermatol, 132: 1933-5.<br/>3. Corssmit EP, Heijligenberg R, Hack CE, et al. 1997. Clin Exp Immunol, 107: 359-63.<br/>4. Corssmit EP, de Metz J, Sauerwein HP, et al. 2000. J Interferon Cytokine Res, 20: 1039-47. | |
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