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Fast, pH-Stable Separation of Oligosaccharides
A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.
Sensitive, direct detection with no derivatization, using pulsed amperometric detection
Very high-efficiency separations
Separations based on size, charge, degree of branching, and linkage isomerism
Low flow rate saves on eluent consumption
Does not require derivatization
CarboPac® PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
| CarboPac PA200 Column for High-Resolution Oligosaccharide Separations Specifications | |
|---|---|
| Dimensions | CarboPac® PA200 Analytical Column: 3 × 250 mm CarboPac PA200 Guard Column: 3 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Temperature range | 6–60 °C Recommended operating temperature: controlled ambient |
| Typical operating conditions | 2700 psi at 0.5 mL/min (analytical and guard columns) Recommended flow rate: 0.5 mL/min |
| phase compatibility | pH 0–14; * compatible with common organic solvents. Ionic form eluents: sodium acetate and sodium hydroxide only. |
| Resin composition | 5.5 µm-diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linked) agglomerated with 43 nm MicroBead® quaternary amine functionalized latex (6% crosslinked) |
| Anion-exchange capacity | Approximay 35 µeq/column (3 × 250 mm analytical column) |
| Column construction | PEEK™ with 10-32 threaded ferrule-style end fittings. All components are nonmetallic |