Recombinant Transforming Growth Factor Beta Induced Protein (TGFbI)

轉(zhuǎn)化生長(zhǎng)因子β誘導(dǎo)蛋白(TGFbI)重組蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Gly423~Leu632

Tags: N-terminal His Tag

Tissue Specificity: Brain, Heart, Liver, Kidney.

Subcellular Location: Secreted, extracellular space, extracellular matrix

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5%trehalose, 0.01%sarcosyl and Proclin300.

Original Concentration: 200µg/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.4

Predicted Molecular Mass: 26.8kDa

Accurate Molecular Mass: 31kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

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