Recombinant Histone Cluster 2, H2ac (HIST2H2AC)  

組蛋白2簇H2ac(HIST2H2AC)重組蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Met1~Lys129

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Lung.

Subcellular Location:  Nucleus. Chromosome.

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration:  200µg/mL

Applications:SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:10.9

Predicted Molecular Mass: 44.0kDa

Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

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