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滬鼎產(chǎn)品文獻(xiàn):植物MgCh,Rubisco,ELISA試劑盒引用文獻(xiàn)
閱讀：1064 發(fā)布時(shí)間：2020-9-30| 提 供 商 | 上海滬鼎生物科技有限公司 | 資料大小 | 1.1MB |
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【文獻(xiàn)標(biāo)題】Transcriptome profiling of genes involved in photosynthesis in Elaeagnus angustifolia L. under salt stress
【作者】J. LIN, J.P. LI, F. YUAN,et.al
【作者單位】山東師范大學(xué)（Shandong Normal University）
【文獻(xiàn)中引用產(chǎn)品】
植物鎂螯合酶(MgCh) ELISA試劑盒
植物Rubisco活化酶(RCA)ELISA試劑盒
【關(guān)鍵詞】biomass; greening; ion concentrations; photosynthetic parameters; plant height.
【DOI】doi.org/10.1007/s11099-018-0824-6
【影響因子(IF)】2.3
【出版期刊】《PHOTOSYNTHETICA》
【產(chǎn)品原文引用】
Protein content and activity of magnesium chelatase and Rubisco:
Magnesium chelatase and Rubisco were prepared by first freezing a 0.2-g leaf sample in liquid nitrogen to prevent proteolytic activity, followed by grinding with 2 mL of extraction buffer (100 mM phosphate buffer, pH 7.2, containing 0.1 mM EDTA, and 2 mM ascorbic acid). The homogenate was centrifuged for 15 min at 10,000 rpm, and the supernatant was used to measure the protein content (ng L–1), magnesium chelatase activity [pmol(Mg-Deutero) min–1 mg–1(protein)] and Rubisco activity [μmol min–1 mg–1(protein)]. Magnesium chelatase and Rubisco were measured using a magnesium chelatase content kit and a Rubisco content kit (double antibody sandwich method, Huding Biological Technology Co., Shanghai, China) (3 replicates per treatment).
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