SuperSignal West Pico Chemiluminescent Substrate  

Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate is an enhanced chemiluminescent （ECL） substrate for horseradish peroxidase （HRP） enzyme that provides low picogram detection of proteins in Western blot applications.

• ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase （HRP） activity from antibodies and other Western blot probes
• Economy – costs less per milliliter than other similarly sensitive chemiluminescent substrates
• Picogram sensitivity – highly sensitive for the rapid development of a wide range of protein levels
• Stable light output – long-duration signal makes it possible to make multiple exposures to film
• High intensity – signal is twice as strong as output from other luminol-based systems
• Excellent stability – 24-hour working solution stability; 1-year kit stability at room temperature
• Saves antibody – requires much less （more dilute） primary and secondary antibodies per blot

Enhance light emission kinetics: Thermo Scientific SuperSignal Substrate vs. GE ECL System. Net relative intensity six hours after incubation is much greater for SuperSignal West Pico Substrate than for the GE ECL System.

Thermo Scientific SuperSignal West Pico is more sensitive than GE ECL Substrate. Recombinant mouse IL-2 was diluted （50 to 0.003ng） and electrophoresis was performed. The gels were transferred to nitrocellulose membranes, blocked and incubated with a 1µg/mL dilution of rat anti-mouse IL-2. After washing, the membranes were incubated with 20ng/mL dilutions of HRP-conjugated goat anti-rat antibody. The membranes were washed again and then incubated with substrate that was prepared according to the manufacturers' instructions. Blots were exposed to film for one- and five-minute exposures

Related Resources: Tech Tip #67: Chemiluminescent Western blotting technical guide and protocols Tech Tip #21: Convert to SuperSignal West Pico Substrate from ECL Substrate Tech Tip #22: Determine source of nonspecific background signal in Western blots Tech Tip #24: Optimize antigen and antibody concentrations for Western blots Related Products: SuperSignal （and other） Western Blotting Kits – include secondary antibodies, etc. Pierce Fast Western Blot Kits with SuperSignal Substrates Selection Guide for Chemiluminescent Western Blot Substrates Blotting Equipment, Membranes and Signal Enhancers CL-XPosure Film （X-ray film for chemiluminescence）

Ordering Information

Product # Description Pkg. Size Instructions MSDS CofA Price 34079 SuperSignal West Pico Trial Kit Formulation: Proprietary luminol and peroxide solutions Sufficient For: 6 mini-gel blots; 500cm2 of membrane Kit Contents: Luminol/Enhancer, 25mL Stable Peroxide Buffer, 25mL 50mL kit Local contact 34077 Formulation: Proprietary luminol and peroxide solutions Sufficient For: 12 mini-gel blots; 1000cm2 of membrane Kit Contents: Luminol/Enhancer, 50mL Stable Peroxide Buffer, 50mL 100mL kit Local contact 34087 Formulation: Proprietary luminol and peroxide solutions Sufficient For: 25 mini-gel blots; 2000cm2 of membrane Kit Contents: Luminol/Enhancer, 100mL Stable Peroxide Buffer, 100mL 200mL kit Local contact 34080 Formulation: Proprietary luminol and peroxide solutions Sufficient For: 60 mini-gel blots; 5000cm2 of membrane Kit Contents: Luminol/Enhancer, 250mL Stable Peroxide Buffer, 250mL 500mL kit Local contact 34080AB Formulation: Proprietary luminol and peroxide solutions Kit Contents: Luminol/Enhancer Stable Peroxide Buffer Custom 34078 Formulation: Proprietary luminol and peroxide solutions Sufficient For: 120 mini-gel blots; 10,000cm2 of membrane Kit Contents: Luminol/Enhancer, 500mL Stable Peroxide Buffer, 500mL 1L kit Local contact